National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Molecular basis of the sperm selection in the mammalian female reproductive tract
Šrámková, Adéla ; Krylov, Vladimír (advisor) ; Postlerová, Pavla (referee)
Internal fertilization is typical for all mammals. The male transfers sperm cells into the female reproductive tract, where usually one of them reach the oocyte and fertilize it. The female reproductive tract must be adapted to provide a suitable environment for the sperm cells to survive until fertilization. On the other hand, it must also be able to select developmentally competent sperm cells and prevent others to get into the close contact with the oocyte. Physical and molecular mutual communication of sperm cells with the female reproductive tract is ensured by sperm surface molecules and the receptors of the female epithelia lining. It appears that these interactions are required for key transformations, both on the sperm surface and in the cells of the female reproductive tract, where gene expression is affected. As a result of these changes, the most suitable sperm cell with all the required characteristics is allowed to fertilize the matured oocyte.
Methods of sperm selection for human and veterinary assisted reproduction
Pavlová, Hana ; Komrsková, Kateřina (advisor) ; Koubek, Pavel (referee)
Sperm selection is an important process influencing the progress of fertilization and the quality of resulting embryos. Competent sperm cells can be isolated from an ejaculate sample according to different criteria. Standard methods allow for sperm selection based on conventional parameters, particularly motility and density. Using advanced methods, sperm cells are selected according to molecular aspects including for instance membrane charge, presence of specific receptors, molecules indicating ongoing apoptosis, and others. The latter procedures are widely used particularly in human practice. Their use is uneconomical in veterinary practice and standard methods including the centrifugation step in the procedure are primarily used to select the sperm of livestock. Sperm cells of livestock are also often selected according to whether it carries the X or Y chromosome. Thus, the sex of the offspring can be effectively determined in order to increase the efficiency of the production of animal products. This thesis presents the most frequently used methods of sperm selection for the purpose of oocyte fertilization in human and veterinary sectors of assisted reproduction and discusses their impact on the parameters and fertilization potential of isolated cells.

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